antibody against wisp1 af1627 Search Results


wisp1 af1627  (R&D Systems)
94
R&D Systems wisp1 af1627
Wisp1 Af1627, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+against+wisp1+af1627/Human+WISP-1%2FCCN4+Antibody/pmc05731897-286-25-28
Average 94 stars, based on 1 article reviews
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ohdg  (Bioss)
96
Bioss ohdg
Ohdg, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+against+wisp1+af1627/8-OHdG+Polyclonal+Antibody/pmc05731897-286-30-34
Average 96 stars, based on 1 article reviews
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resource source identifier antibodies anti wisp1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology resource source identifier antibodies anti wisp1
Figure 2. Reverse translational analysis links <t>WISP1</t> and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.
Resource Source Identifier Antibodies Anti Wisp1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+against+wisp1+af1627/WISP-1+Antibody/pm35987202-230-2-16
Average 93 stars, based on 1 article reviews
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primary human hepatocytes  (ScienCell)
90
ScienCell primary human hepatocytes
Figure 2. Reverse translational analysis links <t>WISP1</t> and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.
Primary Human Hepatocytes, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+against+wisp1+af1627/primary+human+hepatocytes/pm35987202-230-170-173
Average 90 stars, based on 1 article reviews
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primary human hscs  (ScienCell)
90
ScienCell primary human hscs
Figure 2. Reverse translational analysis links <t>WISP1</t> and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.
Primary Human Hscs, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+against+wisp1+af1627/primary+human+hscs/pm35987202-230-157-162
Average 90 stars, based on 1 article reviews
primary human hscs - by Bioz Stars, 2026-10
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primary human hepatic sinusoidal endothelial cells  (ScienCell)
90
ScienCell primary human hepatic sinusoidal endothelial cells
Figure 2. Reverse translational analysis links <t>WISP1</t> and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.
Primary Human Hepatic Sinusoidal Endothelial Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+against+wisp1+af1627/human+hepatic+sinusoidal+endothelial+cells++hhsecs+/pm35987202-230-175-181
Average 90 stars, based on 1 article reviews
primary human hepatic sinusoidal endothelial cells - by Bioz Stars, 2026-10
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1d11  (Jackson Immuno)
96
Jackson Immuno 1d11
Figure 2. Reverse translational analysis links <t>WISP1</t> and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.
1d11, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+against+wisp1+af1627/Normal+Donkey+Serum/pm35987202-230-124-130
Average 96 stars, based on 1 article reviews
1d11 - by Bioz Stars, 2026-10
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wisp1  (R&D Systems)
88
R&D Systems wisp1
Figure 2. Reverse translational analysis links <t>WISP1</t> and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.
Wisp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+against+wisp1+af1627/Human+WISP-1%2FCCN4+Antibody/pm24953558-94-10-12
Average 88 stars, based on 1 article reviews
wisp1 - by Bioz Stars, 2026-10
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Image Search Results


Figure 2. Reverse translational analysis links WISP1 and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.

Journal: Cell metabolism

Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

doi: 10.1016/j.cmet.2022.07.009

Figure Lengend Snippet: Figure 2. Reverse translational analysis links WISP1 and MRTF-cytoskeleton signaling in liver fibrosis (A) Correlation of WISP1 expression with MRTF signature eigengene in fibrotic livers. (B) WISP1 mRNA levels in human livers at distinct fibrosis stage as indicated (MATAVIR F0, n = 12; F1, n = 15; F2, n = 15; F3, n = 14; F4, n = 13). (C) Correlation between WISP1 mRNA levels and liver fibrosis score in human patients described in (B). p value is calculated using Pearson test. (D) WISP1 protein levels in serum samples from patients with NASH (F3/4, n = 32) and healthy volunteers (HV, n = 16). (E) Boxplot showing the percent of cells expressing WISP1 in human cirrhotic livers, split by major cell population, with individual samples shown as diamonds (scRNA-seq data from GEO: GSE136103). (F) Representative images of WISP1 and ACTA2 immunofluorescence in healthy and fibrotic liver sections (scale bars, 25 mm) and quantification of percentage of WISP1/ACTA2 double positive cells. (G) Representative western blots of nuclear (top panel) and total MRTFA and MRTFB (bottom panel) in HSCs stimulated with human WISP1 (hWISP1). Yin Yang 1 (YY1) is a loading control for nuclear fractions. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a loading control for whole-cell lysates. (H) Cytoskeleton gene expression in HSCs transfected with MRTFA and MRTFB or control siRNA in the presence or absence of hWISP1, n = 3 biological replicates. (I and J) Live-cell imaging (I) and quantification (J) of HSC migration toward FBS in the presence or absence of hWISP1. Stellate cells that migrated through pores to bottom surface of transwell were highlighted in yellow. n = 4 biological replicates.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

Techniques: Expressing, Western Blot, Control, Gene Expression, Transfection, Live Cell Imaging, Migration

Figure 3. Wisp1 is required for liver fibrosis progression, but not initiation, in CCl4 model (A) Top 20 GO biological processes enriched in the upregulated genes in livers from 6-week CCl4 model. (B) Heatmap of Wisp1 and cytoskeleton gene expression in livers from 6-week CCl4 model, n = 6 mice/group. (C) Experimental regime of CCl4 model in WT and Wisp1 KO mice at indicated timepoints: 3 weeks (oil/WT, n = 3; CCl4/WT, n = 9; oil/KO, n = 5; CCl4/KO, n = 10) and 6 weeks (oil/WT, n = 4; CCl4/WT, n = 10; oil/KO, n = 4; CCl4/KO, n = 11). (D) Representative trichrome staining images of livers after 3- or 6-week CCl4 treatment; scale bars, 500 mm. (E and F) Quantification of fibrosis by relative lesion size (E) and hydroxyproline (F). (G) Representative western blot of type I collagen and cytoskeleton proteins in liver lysates after 6-week CCl4 treatment. GAPDH is a loading control.

Journal: Cell metabolism

Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

doi: 10.1016/j.cmet.2022.07.009

Figure Lengend Snippet: Figure 3. Wisp1 is required for liver fibrosis progression, but not initiation, in CCl4 model (A) Top 20 GO biological processes enriched in the upregulated genes in livers from 6-week CCl4 model. (B) Heatmap of Wisp1 and cytoskeleton gene expression in livers from 6-week CCl4 model, n = 6 mice/group. (C) Experimental regime of CCl4 model in WT and Wisp1 KO mice at indicated timepoints: 3 weeks (oil/WT, n = 3; CCl4/WT, n = 9; oil/KO, n = 5; CCl4/KO, n = 10) and 6 weeks (oil/WT, n = 4; CCl4/WT, n = 10; oil/KO, n = 4; CCl4/KO, n = 11). (D) Representative trichrome staining images of livers after 3- or 6-week CCl4 treatment; scale bars, 500 mm. (E and F) Quantification of fibrosis by relative lesion size (E) and hydroxyproline (F). (G) Representative western blot of type I collagen and cytoskeleton proteins in liver lysates after 6-week CCl4 treatment. GAPDH is a loading control.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

Techniques: Gene Expression, Staining, Western Blot, Control

Figure 4. WISP1 induces cytoskeleton and motility reprogramming via multiple integrins (A) Representative western blots of hWISP1 induced MRTFA and MRTFB nuclear translocation in HSCs in the presence ofArg-Gly-Asp-Ser (RGDS) or Arg-Gly- Glu-Ser (RGES) peptides. YY1 is a loading control for nuclear fractions, and GAPDH is a loading control for whole-cell lysates. (B) hWISP1 induced cytoskeleton gene expression in the presence of RGDS or RGES peptides in HSCs, n = 3 biological replicates. (C) hWISP1 induced HSC migration in the presence of RGDS or RGES peptides, n = 4 biological replicates. (D–F) hWISP1 induced migration (D), MRTFA and MRTFB nuclear translocation (E), and cytoskeleton gene expression (F) of HSCs transfected with siRNAs tar- geting integrins as indicated, n = 4 (D) or 3 (F) biological replicates. (G and H) RHOA activation (G) and F-actin formation (H) in HSCs stimulated with hWISP1 in the presence of RGDS or RGES peptides, n = 3 (G) or 4 (H) biological replicates. (I and J) RHOA activation (I) and F-actin formation (J) in HSCs transfected with siRNAs targeting integrins as indicated, n = 3 (I) or 4 (J) biological replicates. Data in (B)–(J) except (E) represent mean ± SEM, and similar results were seen in at least three independent experiments. p value is calculated using: (C, F, I, and J) two-way ANOVA and (B, D, G, and H) one-way ANOVA. See also Figure S9.

Journal: Cell metabolism

Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

doi: 10.1016/j.cmet.2022.07.009

Figure Lengend Snippet: Figure 4. WISP1 induces cytoskeleton and motility reprogramming via multiple integrins (A) Representative western blots of hWISP1 induced MRTFA and MRTFB nuclear translocation in HSCs in the presence ofArg-Gly-Asp-Ser (RGDS) or Arg-Gly- Glu-Ser (RGES) peptides. YY1 is a loading control for nuclear fractions, and GAPDH is a loading control for whole-cell lysates. (B) hWISP1 induced cytoskeleton gene expression in the presence of RGDS or RGES peptides in HSCs, n = 3 biological replicates. (C) hWISP1 induced HSC migration in the presence of RGDS or RGES peptides, n = 4 biological replicates. (D–F) hWISP1 induced migration (D), MRTFA and MRTFB nuclear translocation (E), and cytoskeleton gene expression (F) of HSCs transfected with siRNAs tar- geting integrins as indicated, n = 4 (D) or 3 (F) biological replicates. (G and H) RHOA activation (G) and F-actin formation (H) in HSCs stimulated with hWISP1 in the presence of RGDS or RGES peptides, n = 3 (G) or 4 (H) biological replicates. (I and J) RHOA activation (I) and F-actin formation (J) in HSCs transfected with siRNAs targeting integrins as indicated, n = 3 (I) or 4 (J) biological replicates. Data in (B)–(J) except (E) represent mean ± SEM, and similar results were seen in at least three independent experiments. p value is calculated using: (C, F, I, and J) two-way ANOVA and (B, D, G, and H) one-way ANOVA. See also Figure S9.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

Techniques: Western Blot, Translocation Assay, Control, Gene Expression, Migration, Transfection, Activation Assay

Figure 5. Discovery of a WISP1-blocking antibody with therapeutic benefits (A and B) Biacore sensorgram plots of anti-WISP1 binding to hWISP1 (A) or mWISP1 (B). (C) Dose-dependent inhibitory activity of anti-WISP1 against hWISP1 induced HSC migration, n = 4 biological replicates.

Journal: Cell metabolism

Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

doi: 10.1016/j.cmet.2022.07.009

Figure Lengend Snippet: Figure 5. Discovery of a WISP1-blocking antibody with therapeutic benefits (A and B) Biacore sensorgram plots of anti-WISP1 binding to hWISP1 (A) or mWISP1 (B). (C) Dose-dependent inhibitory activity of anti-WISP1 against hWISP1 induced HSC migration, n = 4 biological replicates.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

Techniques: Blocking Assay, Binding Assay, Activity Assay, Migration

Figure 6. Therapeutic benefits of WISP1 blocking antibody in CDA-HFD NASH model (A) Unsupervised hierarchical clustering analysis of 25-gene NASH fibrosis signature gene expression in livers from mice fed with normal chow and CDA-HFD diet (8 weeks and 16 weeks). (B) Dosing regime in the preventive (weeks 0–8) and therapeutic (weeks 9–16) CDA-HFD NASH model: n = 10 mice for each group. (C) Representative Sirius red staining images of livers; scale bars, 250 mm. (D and E) Quantification of fibrosis by relative lesion size (D) and newly synthesized hydroxyproline (E). (F) Representative western blots of type I collagen and cytoskeleton proteins in liver lysates from the therapeutic CDA-HFD NASH model. GAPDH is a loading control. (G and H) Representative images (G) and quantification (H) of ACTA2 immunofluorescence in livers; scale bars, 100 mm. Data in (D), (E), and (H) represent mean ± SEM. p value is calculated using one-way ANOVA. See also Figure S11.

Journal: Cell metabolism

Article Title: A WISP1 antibody inhibits MRTF signaling to prevent the progression of established liver fibrosis.

doi: 10.1016/j.cmet.2022.07.009

Figure Lengend Snippet: Figure 6. Therapeutic benefits of WISP1 blocking antibody in CDA-HFD NASH model (A) Unsupervised hierarchical clustering analysis of 25-gene NASH fibrosis signature gene expression in livers from mice fed with normal chow and CDA-HFD diet (8 weeks and 16 weeks). (B) Dosing regime in the preventive (weeks 0–8) and therapeutic (weeks 9–16) CDA-HFD NASH model: n = 10 mice for each group. (C) Representative Sirius red staining images of livers; scale bars, 250 mm. (D and E) Quantification of fibrosis by relative lesion size (D) and newly synthesized hydroxyproline (E). (F) Representative western blots of type I collagen and cytoskeleton proteins in liver lysates from the therapeutic CDA-HFD NASH model. GAPDH is a loading control. (G and H) Representative images (G) and quantification (H) of ACTA2 immunofluorescence in livers; scale bars, 100 mm. Data in (D), (E), and (H) represent mean ± SEM. p value is calculated using one-way ANOVA. See also Figure S11.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-WISP1 This paper N/A Anti-WISP1 R&D Cat#AF1627; RRID: AB_2216596 Anti-MRTFA Santa Cruz Cat#sc-32909; RRID: AB_2142495 Anti-MRTFA Sigma-Aldrich Cat#HPA030782; RRID: AB_10600930 Anti-MRTFB Santa Cruz Cat#sc-98989; RRID: AB_2250525 Anti-ACTA2-FITC Sigma-Aldrich Cat#F3777; RRID: AB_476977 Anti-ACTA2 Abcam Cat#ab5694; RRID: AB_2223021 Anti-PDGFRß R&D Cat#AF1042; RRID: AB_2162633 Anti-YY1 Abcam Cat#ab109237; RRID: AB_10890662 Anti-COL1 Abcam Cat#ab21286; RRID: AB_446161 Anti-FLNA GeneTex Cat#112939; RRID: AB_2036932 Anti-CFL1 Cell Signaling Cat#5175; RRID: AB_10622000 Anti-MYL9 Cell Signaling Cat#8505; RRID: AB_2728760 Anti-TPM4 Abcam Cat#ab181085; RRID: AB_2205772 Anti-GAPDH Cell Signaling Cat#5174; RRID: AB_10622025 Anti-ACTB Sana Cruz Cat#sc-47778; RRID: AB_626632 Anti-CDH1 Cell Signaling Cat#3195; RRID: AB_2291471 Anti-FN1 Sigma-Aldrich Cat#F3648; RRID: AB_476976 Anti-SNAI1 Cell Signaling Cat#3895; RRID: AB_2191759 Anti-aVb5 integrin R&D Cat#MAB2528; RRID:AB_2280706 Anti-a5b1 integrin This paper N/A Anti-GP120 This paper N/A Anti-TGFb Home-made 1D11; RRID: AB_935663 Normal donkey serum Jackson Immuno Research Cat#017-000-121; RRID: AB_2337258 Biological samples Healthy and NASH liver tissues from human patients LifeNet health N/A Serum samples from human patients with NASH This paper N/A Primary human HSCs ScienCell Cat#5300 Primary human Kupffer cells Lonza Cat#HLKC Primary human hepatocytes ScienCell Cat#5200 Primary human hepatic sinusoidal endothelial cells ScienCell Cat#5000 Primary human lung fibroblasts This paper N/A Chemicals, peptides, and recombinant proteins Corn oil Sigma-Aldrich Cat#C8267 Carbon tetrachloride (CCl4) Sigma-Aldrich Cat#319961 Deuterated water Cambridge Isotope Laboratories DLM-4-99.8-1000 Recombinant human WISP1 R&D Cat#1627-WS-050 Recombinant mouse WISP1 R&D Cat#1680-WS-050 Recombinant PDGF-BB R&D Cat#220-BB Recombinant CCL2 R&D Cat#279-MC Recombinant VEGF R&D Cat#293-VE Mitomycin C Sigma-Aldrich Cat#M4287 (Continued on next page) e1 Cell Metabolism 34, 1377–1393.e1–e8, September 6, 2022

Techniques: Blocking Assay, Gene Expression, Staining, Synthesized, Western Blot, Control